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ARCHIVE jun days apr days mar days feb days jan days dec days nov days oct days sept days jul days jun days may days mar days feb days jan days ARCHIVE dec days nov days oct days sept days aug days jun days may days | Osamu Shimomura/Marine Biological Laboratory, | green fluorescent protein image on the left Osamu Shimomura, Martin Chalfie and Roger Y. Tsien won the 2008 Nobel Prize in Chemistry (10/8/08) for taking the ability of the jellyfish to glow green and transforming it into a tool of molecular biology to watch the dance of living cells and the proteins within them. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Question: Is the green fluorescent color a form of light or from pigment (dye)? – crb Answer: It's definitely a form of light. Fluorescence means some light hits the (in this case) protein, which then re-emits the light at a different (longer) wavelength. In this case the emitted light is green. It actually shoots out photons like a little flashlight. Probably you'd shine essentially white light on it (from sunlight or from artificial lights) and it would light up green. This is very different from a dye, which absorbs most of the light that hits it, and reflects back what it doesn't absorb. This is a "subtractive" process. The photons that you see reflected were already present in the incident light. With fluorescence the photon you see was created de novo in the protein using the energy of the incident light. – Joel Cohen, Biophysicist | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Photo released by the University of California | Image on left “It's not something out of the blue, but you never know when it's going to come or if it's going to come, so it's always a big surprise when it actually happen.”
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AP Photo/Harvard University, Livett- Weissman-Sanes-Lichtman | image on the left As a child Roger Y. Tsien was dazzled by colors, and that later helped him in his career. He said, “I feel a bit like a deer caught in headlights.” “I like pretty colors . . .this was a good opportunity. “A deer caught in headlights.” – the effects on your rod cells being overwhelmed before your cone cells can adjust to the bright light. — crb | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
AP Photo/Harvard University, Livett- Weissman-Sanes-Lichtman |
When exposed to ultraviolet light, the protein glows green. It can act as a marker on otherwise invisible proteins within cells to trace them as they go about their business. It can tag individual cells in tissue. And it can show when and where particular genes turn on and off.
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